Improving diagnostics is an important part of identifying pathogens and enhancing antimicrobial stewardship. Identification of the pathogen causing the disease will lead to more effective vaccination programs including in some circumstances the ability to use an autogenous vaccine when no other solution is available.
This paper recently published in Journal of Microbiological Methods, titled An improved multiplex PCR for Actinobacillus pleuropneumoniae, Glaesserella australis and Pasteurella multocida was co-authored by Apiam swine vet Dr Sarah Jenkin.
Glaesserella australis, a newly described bacterial species, has been isolated from pig lungs that displayed lesions
very similar to those caused by Actinobacillus pleuropneumoniae, prompting the need for a validated diagnostic
tool. In this work, the authors have altered a multiplex PCR used for the identification of cultures of G. australis,
A. pleuropneumoniae and Pasteurella multocida to be more sensitive and then evaluated the use of the altered
diagnostic tool on cultures and directly on tissues. The altered multiplex PCR was validated using 47 related
species, both type/reference strains and field isolates.
Authors:
Xiaochen Sun a, P.J. Blackall a, Patrick Daniel b, Kerri Chandra c, Sarah Jenkin d, Conny Turni a,*
a Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland 4067, Australia
b Pig Services Centre, Agriculture, Food and Fibre Division, Department of Jobs, Precincts and Regions, Epsom, Victoria 3551, Australia
c Department of Agriculture and Fisheries, Ecosciences Precinct, Dutton Park, Queensland 4102, Australia
d Apiam Animal Health, Bendigo, Victoria 3550, Australia
